Journal: ACS Biomaterials Science & Engineering

Article Title: Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues

doi: 10.1021/acsbiomaterials.1c01193

Figure Lengend Snippet: Conductivity and biocompatibility of GelMA-AuNPs and GelMA-MXene hydrogels. (A) Conductivity of pure GelMA, GelMA-AuNPs, and GelMA-MXene hydrogels; (B) cell viability; and (C) fluorescent microscopy images of C2C12 cells encapsulated in pure GelMA, GelMA-AuNPs, and GelMA-MXene hydrogels. Red cells are dead cells, while green cells are live cells.

Article Snippet: The murine myoblast cell line, C2C12, was obtained from Addexbio Technologies.

Techniques: Microscopy

Journal: ACS Biomaterials Science & Engineering

Article Title: Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues

doi: 10.1021/acsbiomaterials.1c01193

Figure Lengend Snippet: Bioprinted C2C12 using GelMA-AuNPs and GelMA-MXene bioinks. (A) Fluorescent microscopy images of cells bioprinted using GelMA-AuNPs and GelMA-MXene hydrogels. Red cells are dead cells, while green cells are live ones. (B) Viability of cells encapsulated in GelMA, GelMA-AuNPs, and GelMA-MXene hydrogels printed and nonprinted (bulk) on days 1 and 7. (C) Cells encapsulated in GelMA-AuNPs hydrogels bioprinted to produce different structures.

Article Snippet: The murine myoblast cell line, C2C12, was obtained from Addexbio Technologies.

Techniques: Microscopy

Journal: ACS Biomaterials Science & Engineering

Article Title: Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues

doi: 10.1021/acsbiomaterials.1c01193

Figure Lengend Snippet: Differentiation of C2C12 cells encapsulated in pure GelMA, GelMA-AuNPs, and GelMA-MXene hydrogels. (A) Fluorescent microscopy images of cells encapsulated in GelMA-AuNPs and GelMA-MXene hydrogels and stained for the myosin heavy chain (MHC), (B) fusion index, and (C) myotube length and diameter for C2C12 cells encapsulated in pure GelMA, GelMA-AuNPs, and GelMA-MXene hydrogels.

Article Snippet: The murine myoblast cell line, C2C12, was obtained from Addexbio Technologies.

Techniques: Microscopy, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: HIF-1α Directly Controls WNT7A Expression During Myogenesis

doi: 10.3389/fcell.2020.593508

Figure Lengend Snippet: Characterization of WNT7A inhibition using CRISPR/Cas9 model in C2C12. (A) Graphical representation of the mouse WNT7A -targeted locus. Boxes and lines indicate exons and introns, respectively. The oligo represents sgRNA target sequence and Protospacer Adjacent Motifs (PAM) site is marked in red. (B) Sanger DNA sequencing were conducted on PCR products amplified from the genomic WNT7A loci of C2C12. (C,D) Genomic PCR analysis (C) and quantification of WNT7A inhibition (D) . RPLI was used as the housekeeper ( n = 3). (E,F) Western Blot analysis (E) and quantification (F) of WNT7A expression in wild type (WT) and WNT7A -silenced murine myoblasts ( WNT7A -KO; n = 3). (G) Cell viability analyzed by RealTime-Glo TM kits MT Cell Viability Assay of wild type and WNT7A -silenced murine myoblasts ( n = 3). Data information: all data represent mean ± SD. * p < 0.05, **** p < 0.0001 (Student’s t -test).

Article Snippet: C2C12 murine myoblasts (Merck), HIF-1α-silenced C2C12 murine myoblasts (shHIF-1α), and WNT7A-KO silenced C2C12 murine myoblasts, which were obtained in our previous study , were cultured in growth medium (GM) made of Dulbecco’s modified Eagle’s medium (DMEM, Merck) with high glucose concentration (4.5 g/L) supplemented with 10% (v/v) fetal bovine serum (FBS, Merck), 2 mM glutamine (Merck), penicillin/streptomycin 1X (Euroclone; GM) at 37°C in a 5% CO 2 and 95% air-humidified atmosphere.

Techniques: Inhibition, CRISPR, Sequencing, DNA Sequencing, Amplification, Western Blot, Expressing, Viability Assay